The outstanding technique of gene cloning is largely dependent on vectors. Vector is a DNA molecule which is used for the transfer of foreign genetic material from one cell to another and, hence, also known as cloning vehicle. It has the capability to steadily maintain itself and replicate in an appropriate host organism.
A typical vector consists of the following components:
· Origin of replication
The origin of replication present in the vector aids its efficient replication inside the host cells. During this process, the cloned DNA fragment in the vector gets replicated too. This results in the amplification of the cloned DNA fragment and hence any subsequent manipulations and analysis can be easily done. Moreover, the stored vector-DNA fragment construct can be further used to produce indefinite amounts of the DNA fragment.
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