Pages

▼
February 9, 2011

Ampicillin

›
Ampicillin is one of the most widely used antibiotic in molecular cloning, for the selection of transformants. It belongs to the penicillin...
February 5, 2011

7 Factors affecting expression of heterologous proteins in E. coli

›
The gram negative bacterium, E. coli is a versatile and valuable organism for the high-level expression of heterologous proteins. This is m...
1 comment:
February 1, 2011

RNA isolation Protocol

›
Isolation of the high quality RNA is the first step for numerous molecular biology experiments. These experiments can be RT-PCR, Real-Time ...
2 comments:
January 30, 2011

9 Factors affecting DNA extraction from agarose gel

›
           Agarose gel electrophoresis is a method of choice for the identification, purification, and separation of the DNA fragments. DNA...
1 comment:
January 27, 2011

Choosing the right DNA polymerase for your PCR

›
Success of the polymerase chain reaction (PCR) largely depends on the choice of the appropriate DNA polymerase. DNA polymerase is one of th...
January 24, 2011

Three commonly used affinity tags for protein purification

›
In order to purify heterologous proteins from various hosts, affinity tags are extremely capable tools. In the recent years affinity tags ha...
January 22, 2011

RNA Isolation: Principle and Procedure

›
In order to perform various fundamental molecular biology experiments, the first and most crucial step is isolating high quality, intact RN...