The gram negative bacterium, E. coli is a versatile and valuable organism for the high-level expression of heterologous proteins. This is mainly because the bacterium is easy to genetically manipulate, inexpensive to culture and expression of recombinant proteins is comparatively fast.
However, in spite of all these features, all recombinant proteins are not efficiently expressed in this organism. This may be due to certain factors including the affinity tag used, size and source of the heterologous protein, codon biasing, E. coli strain used, culture conditions and protein degradation affecting the expression of recombinant protein. Here you will find the details of these factors affecting the expression of foreign proteins in the bacterium.
1 comment:
Hi,
Protein expression is a subcomponent of gene expression. The bacterium is easy to genetically manipulate, inexpensive to culture and expression of recombinant proteins is comparatively fast. Thanks for sharing this information.
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