Extraction of DNA from agarose gel

The extraction of DNA molecules from agarose gels is routinely employed for the downstream processing of the DNA fragments. Gel extraction is frequently done for the cloning, radio-labeling and sequencing of the DNA molecules. Generally, the purification of DNA fragments involves the separation of DNA from agarose gel slices.

            The reaction components of certain molecular cloning techniques e.g. a PCR amplification or restriction enzyme digestion, include certain proteins and salts that may inhibit further applications of the DNA fragment of interest. Hence, in order to use the DNA fragments for additional purpose, these components musts be removed. It has been observed that purification of DNA generally results in the improvement of its efficiency in subsequent processing. For example, the digested DNA can be ligated and the PCR products can be directly cloned into the T/A cloning vector. 

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