RNA Isolation: Principle and Procedure

In order to perform various fundamental molecular biology experiments, the first and most crucial step is isolating high quality, intact RNA. These experiments include Northern hybridization, RT-PCR, RNA mapping, nuclease protection assays, in vitro translation, cDNA library construction and so on.

 In a cell, 80-85% of the total RNA is contributed by ribosomal RNA (rRNA). However, messenger RNA (mRNA) is only 1-5% of the total cellular RNA. 

[Read More...]