PCR

PCR is one of the most basic techniques having a major impact on various aspects of molecular biology. It provides massive and highly specific amplification of a precise fragment of DNA whose short sequences of either ends are known. Conceptually PCR is the oldest and practically it is the most versatile tool.

The concept of PCR dates back to the early seventies of the last century. In 1971, H.G. Khorana along with his colleagues, proposed a strategy for the chemical synthesis of the DNA. The method was the same as of now-a-days PCR. Unfortunately, due to lack of availability of many important factors it didn’t seem practical and hence was forgotten. 

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